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Payload

As explained in Acing the CCNA Exam: Volume 1 Fundamentals and Protocols

A payload is the data carried inside a protocol data unit, excluding that unit's protocol control information.

A payload is the contents carried inside a protocol data unit (PDU), excluding that unit’s protocol control information, such as its header or trailer. The payload changes meaning as data moves through the TCP/IP layers. section 4.3.2

Track the nested payloads during encapsulation:

Following data through the layers

A host adds a Layer 4 header to application data, creating a segment; adds a Layer 3 header, creating a packet; then adds a Layer 2 header and trailer, creating a frame. At the destination, those control fields are inspected and removed in reverse order until the application receives the data. section 4.3.2 section 4.4

Figure 4.7 Application data encapsulated in a Layer 4 header is a segment (L4PDU); a segment encapsulated in a Layer 3 header is a packet (L3PDU); and a packet encapsulated in a Layer 2 header/trailer is a frame (L2PDU). The encapsulated contents of each PDU are that PDU’s payload.

Use the PDU diagram to see how each layer’s unit becomes the next layer’s payload. section 4.3.2

How Acing the CCNA Exam: Volume 1 Fundamentals and Protocols covers it

How deep it goes
Defines nested payloads and follows their wrapping and removal, but stops short of protocol-specific field analysis.
Examples
Uses application data, segments, packets, and frames to illustrate progressively nested protocol data units.
What it assumes
Assumes basic TCP/IP layer terminology while defining segments, packets, frames, and protocol data units.
What you can do after
Evaluate a segment, packet, or frame by identifying which encapsulated contents form its payload.
How it connects
Connects payload with protocol data units, encapsulation, de-encapsulation, segments, packets, and frames.

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This explainer was generated by Manning's knowledge graph from the text of Acing the CCNA Exam: Volume 1 Fundamentals and Protocols. The section links point to the parts of the book it draws on.